Technical Data

C0115-08B
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Isotype
IgG
Clone Number
5G25
Grade
Supernatant
Applications
IHC
Crossreactivity
Ca Eq Hu Mk Mo Po Rt Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Calcitonin

Calcitonin is a 32aa peptide which can be demonstrated in C cells of the normal and hyperplastic thyroid. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.

Applications
Suitable for use in Immunohistochemistry. Other aplications not tested.
Recommended Dilutions
Immunohistochemistry (Formalin/paraffin): 1:200 for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues. Optimal dilutions to be determined by the researcher.
Recommended Positive Control
Thyroid or medullary carcinoma
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa1-32 of human calcitonin.|Cellular Localization: Cytoplasmic.
Form
Supplied as a liquid in 0.09% sodium azide.
Purity
Supernatant
Specificity
Recognizes human Calcitonin. Species Crossreactivity: monkey, mouse, rat, canine, equine, sheep and porcine

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Spieker-Polet, H., et al., Proc. Natl. Acad. Sci. 92: 9348-9352 (1995). 2. Liguori, M.J., et al., Hybridoma 20: 189-198 (2001). 3. Cano, G., et al., Diagn. Cytopathol. 29: 207-211 (2003). 4. Diaz, L.K. & Sneige, N., Adv. Anat. Pathol. 12: 10-19 (2005). 5. Huang, Z., et al., Appl. Immunohistochem. Mol. Morphol. 13: 91-95 (2005). 6. Rossi, S., et al., Modern Pathology 17(1): 361A (2004). 7. Blechner, M., et al., Modern Pathology 17(1): 241A (2004). 8. Cheuk, W., et al., Am. J. Surg. Path. 28: 801-807 (2004). 9. Budd, G.B., et al., Abstract presented at ASCO Annual meeting, June 2004, New Orleans. 10. Tarr, S.M., et al., Abstract presented at Association for Molecular Pathology meeting, Los Angeles, 2004. 11. Gown, A.M., et al., Modern Pathology 18(1): 35A (2005). 12. Treaba, D.O., et al., Modern Pathology 18(1): 53A (2005). 13. Rossi, S., et al., Am. J. Clin. Pathol. 124: 295-302 (2005).
USBio References
No references available
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