Rabbit Anti-Calmodulin-Dependent Protein Kinase II beta (CAMKIIb, MGC29528) (Not for Export EU)
The multifunctional Ca2+/ calmodulin dependent protein kinase II also known as Calcium/calmodulin Kinase (CaMK II) is a well known effector of calcium and calmodulin-mediated functions. It is present in many tissue but it is abundant in brain. CaMKII also required for synaptic plasticity such as Long Term Potentiation (LTP), a cellular model for learning and memory.
Applications
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:5000. Immunocytochemistry: 1:100. Immunofluorescence: 1:100. (Staining can be weak.) Optimal dilutions to be determined by the researcher.
Positive Controls
Human, mouse or rat brain
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to amino acids 499-513 (VRRGSGTPEAEAPRQ) of human CAMKIIb; Gene ID: 816 CAMK2B.
Form
Supplied as a liquid, 0.05% sodium azide, 50% glycerol.
Specificity
Recognizes human CAMKIIb at ~68kD. Species Crossreactivity: mouse and rat.
