C1103-05
Clone Type
PolyclonalHost
GoatSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IP WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CGoat Anti-Carbohydrate Sulfotransferase 5 (CHST5, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5, ALYE870, FLJ22167, GlcNAc6ST-3, GST4-alpha, hIGn6ST, I-GlcNAc6ST, Intestinal GlcNAc-6-sulfotransferase, MGC74625, PRO1886)
CHST5 (Carbohydrate sulfotransferase 5; also GlcNAc6ST-3, I-GlcNAc6ST and GST4 alpha) is likely a 53-56kD member of the sulfotransfease family of enzymes. It is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium. It appears to act only on short carbohydrates and transfers sulfate to position #6 of mucin-associated GlcNAc. Human CHST5 is a 390aa type II transmembrane protein. It contains a short cytoplasmic segment (aa1-9) and a long luminal domain (aa28-390) that contains an enzymatic region (aa180-358). There is one alternate start site that is 22aa upstream of the standard site. Humans have a GST4-alpha and -beta gene, the result of gene duplication. Over aa28-390, these two gene products share 87% aa identity. Mouse has only one GST4 gene, and it is functionally related to human GST4- beta. Over aa 28-390, human GST4-alpha/CHST5 is 78% aa identical to mouse GST4.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
CHO cell-derived, recombinant human CHST5 lumenal domain aa28-390; Q9GZS9.
Form
Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CHST5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. deGraffenried, D. and Bertozzi, C.R. (2003) J. Biol. Chem. 278: 40282.|2. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.|3. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.|4. Bistrup, A. et al. (1999) J. Cell Biol. 145:899.|5. Uchimura, K. et al. (2002) J. Biol. Chem. 277: 3979.|6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V,|Academic Press, Inc., New York, 964.|7. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613.|8. Wu, Z.L., et al. (2002) Faseb J. 16:539.USBio References
No references available