C2069-44-APC
Clone Type
PolyclonalHost
RabbitSource
MouseConjugate
APCIsotype
IgGGrade
Affinity PurifiedApplications
FLISA IP WBCrossreactivity
Ch Hu Mo Rt XeShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
BSA Free
Rabbit Anti-a-Catenin (APC)
Adherens junctions (AJ) (also referred to as zonula adherens) are required for both the establishment and maintenance of epithelial layers (2). In addition, these junctions have been identified in several other cell types including cardiac myocytes and fibroblasts (2). AJ subserve several important functions including: mediating intercellular adhesion, sensing the presence of neighboring cells, and anchoring the actin cytoskeleton (2,3). AJ are multiprotein complexes that are assembled around cell adhesion molecules called cadherins. Cadherins are a multifunctional family of Ca2+-dependent, transmembrane, glycoproteins which promote cell-cell adhesion (4). The cadherin extracellular domain mediates homophilic interactions between like cadherin molecules on neighboring cells, while the intracellular domain interacts with several cytoplasmic proteins which include: a-catenin, b-catenin, g-catenin (plakoglobin), and the tyrosine kinase substrate p120 cas (1,5,6,7,8,9). Cadherin-catenin interactions are required for complete cadherin activity and regulate the interaction between cadherins and the actin-based cytoskeleton (1,4,6,8,9,10). In fact, deletion of the cadherin cytoplasmic domain produces an adhesion-defective molecule that is unable to interact with the cytoskeleton (4,11).
Applications
Suitable for use in FLISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
FLISA: 0.1-1ug/ml Western Blot: 1-3ug/ml Immunoprecipitation: 5-15ug Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide derived from the C-terminus of mouse a-Catenin.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by immunoaffinity chromatography.
Specificity
Specific for a-catenin, a 102kD protein. Does not crossreact with the related b-catenin or g-catenin proteins. |Species Crossreactivity: Human, Mouse, Rat, Chicken, Xenopus. Tissues/Lysates Tested: HeLa cells, A431 cells, WI-38 human fibroblasts, Xenopus Cytosol, NIH 3T3 cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Herrenknecht, K., et al., Proc. Natl. Acad. Sci. USA 88: 9156-9160 (1991). 2. Geiger, B., et al., Curr. Opin. Cell Biol. 1: 103-109 (1989). 3. Peifer, M., et al., Science 262: 1667-1668 (1993). 4. Takeichi, M., et al., Science 51: 1451-1455 (1991). 5. Daniel, J.M., et al., Mol. Cell. Biol. 270: 4819-4824 (1995). 6. Hinck, L, et al., J. Cell. Biol. 125: 1327-1340 (1994). 7. Knudsen, K.A., et al., J. Cell. Biol. 18: 671-679 (1992). 8. McCrea, P.D., et al., J. Biol. Chem. 266: 4514-4520 (1991). 9. Ozawa, M., et al., Proc. Natl Acad. Sci. USA. 87: 4246-4250 (1990). 10. Knudsen, K.A., et al., J. Biol. Chem. 130: 67-77 (1995). 11. Ozawa, M., et al., J. Cell. Biol. 116: 989-996 (1992). 12. Nagafuchi, A., et al., Cell 65: 849-857 (1991). 13. Nagafuchi, A., et al., J. Cell Biol. 127: 235-245 (1994). 14. McGregor, et al., Biochem. J. 301: 225-233 (1994). 15. Menkel, A.M., et al., J. Cell Biol. 126: 1231-1240 (1994). 16. Rimm, D.L., et al., Proc. Natl. Acad. Sci. USA 92: 8813-8817 (1995). 17. St. Croix, B., et al., J. Cell Biol. 142(2): 557-571 (1998).USBio References
No references available