Mouse Anti-Casein Kinase 1 alpha (CK1a)
Caseine Kinase 1 alpha belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily. CK1 isoforms (alpha, beta, gamma, delta, epsilon) and their splice variants are involved in diverse cellular processes including membrane trafficking, circadian rhythm, cell cycle progression, chromosome segregation, apoptosis and cellular differentiation. Perhaps it can phosphorylate a large number of proteins and is involved in Wnt signaling where it phosphorylates CTNNB1 on Ser45. Caseine kinase 1 alpha also interacts with the Axin complex
Applications
Suitable for use in ELISA, Western Blot and Immunofluorescence/Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunofluorescence (IC): 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant human Casein Kinase 1 alpha (aa1-337) purified from E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Casein Kinase 1alpha.
