Mouse Anti-Caspase 3 (CPP-32, Apoptain, Yama, SCA-1) (MaxLight 650)
MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Caspases are a family of cysteine proteases that centrally controls apoptotic machinery. Caspases can be grouped according to their substrate specificities that are largely determined by the amino acids preceding the cleavage site. One group of caspases that include -6, -8, (MACH/FLICE), and –9(V/LEXD) is specific for the substrate V/LEXD. This substrate is a site similar to those found in caspase proenzymes. This group of caspases may function as initiators of a proteolytic cascade by activating pro-caspases to amplify a death signal. A second group of caspases (-2, -3 and -7) is specific for the substrate DEXD that is related to sites found on target proteins cleaved during apoptosis. This suggests that this second group of caspases function during the effector phase of cell death. Protolytic proteases activate caspases that are expressed as inactive precursors. However, the caspase cascade is not yet well defined, and some caspases may function both upstream and downstream. The Bcl-2 protein inhibits apoptosis and is cleaved at ASP-34 by caspases during apoptosis and by recombinant caspase-3 in vitro. The cleavage of Bcl-2 and caspase-7 is abolished during the immunodepletion of caspase-3, however, the immunodepletion of caspase-7 has no effect on Bcl-2 cleavage. Caspase-3-dependent cleavage of Bcl-2 may promote further caspase activation as part of a positive feedback loop for apoptosis. (1)
Applications
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 1-2ug/ml Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Full length, recombinant human Caspase 3 protein.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Purity
Purified by Protein A affinity chromatography from ascites.
Specificity
Specific to human caspase 3. Reactivity is confirmed with human 293 and Jurkat cell lysates.
