Rabbit Anti-Caspase 9 (ICE-LAP6, Mch6, Apaf 3) (Not for Export EU)
Caspase 9 is a member of the Ced3/ICE cysteine protease caspase family that has a predicted molecular mass of 46kD and was originally isolated in human Jurkat T cells. Caspase 9 is critical for cytochrome c-dependent apoptosis and normal brain development. The active form of caspase 9 is composed of a 32-35kD subunit and a 10kD subunit that are proteolytically derived from procaspase 9 by caspase 3 (CPP3) and granzyme B. Procaspase 9 activation involves formation of a complex with the cofactor Apaf-1, cytochrome C, and deoxyadenosine triphosphate. Once activated, caspase 9 initiates a caspase cascade involving caspase 3,-6, and-7. Caspase 9 is expressed in a variety of tissues. The small heat shock protein Hsp27, has been shown to inhibit etoposide-induced apoptosis by preventing cytochrome C and dATP-triggered activity of caspase-9.
Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:2000 Immunoprecipitation: 1:150 Immunohistochemistry (Paraffin): 1:5000 Immunocytochemistry: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa112-130, PEIRKPEVLRPETPRPVD from human Caspase 9.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity
Recognizes human Caspase 9. Detects both Procaspase 9 and the 35kD large subunit of activated Caspase 9. Recognizes the endogenous protein by Immunohistochemistry and Western Blot. Species Crossreactivity: canine, mouse and rat
