Goat Anti-CCL26-Like (CCL26L, Eotaxin-3 Like)
Chemokine (CC motif) ligand 26, also known as eotaxin 3, is a human CC chemokine ligand that binds and signals via the chemokine receptor CCR3. Rat CCL26-like/Eotaxin 3-like is an 8.5kD orthologue of the human CCL26/eotaxin 3. It is a 94 amino acid (aa) residue protein with a 23 aa signal sequence and a 71 aa mature segment. It is presumed that rat CCL26-like/Eotaxin 3-like will bind CCR3 to induce eosinophil chemotaxis and transendothelial migration. Mature rat CCL26 shares 48%, 63%, and 48% aa sequence identity with the human, mouse and canine CCL26/Eotaxin 3, respectively.
Applications
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect rat CCL26-like. The detection limit for rrCCL26-like is approximately 1 ng/lane under non-reducing and reducing conditions, respectively. Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect rat CCL26-like. The detection limit for rrCCL26-like is approximately 0.5ng/well. Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-derived recombinant rat CCL26-like/Eotaxin‑3-like, Thr24-Trp94.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Selected for its ability to recognize rat CCL26L in direct ELISAs and Western Blots. In these formats, this antibody shows less than 1% crossreactivity with rmCCL26-like.
