Rat Anti-CCR7 (Chemokine Receptor 7, CC-CCR-7)
CCR7 is a 7TM G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Mouse CCR7 shares 87% amino acid sequence identity with human CCR7.
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Neutralization. Other applications have not been tested.
Recommended Dilution
Flow Cytometry: 25ug/ml; 10ul of the diluted solution labels 1-2.5x10e5 cells in a total volume ≤ 200ul. Immunohistochemistry: 25ug/ml stains paraformaldehyde-fixed mouse splenocytes. Neutralization: The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of C2099-71M was determined to be approximately 2-10ug/ml in the presence of 25ng/ml of rmMIP-3beta using the BaF/3 mCCR7 cell line in a chemoattraction assay. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to mouse CCR7 expressed in RBC-2H3 cells (NP_031745).
Form
Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography. |Endotoxin: <0.1EU/ug (LAL)
Specificity
Recognizes mouse CCR7.
