C2099-96B
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1,kClone Number
T21/8Grade
Affinity PurifiedApplications
E FC IC IP WBCrossreactivity
HuAccession #
NM_000579, NP_000570.1Gene ID
1234Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-CD195 (C-C Chemokine Receptor Type 5, C-C CKR-5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 Fusion Coreceptor, CD195, CMKBR5)
CD195 / CCR5 (also known as CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 alpha, MIP-1 beta, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 / CCR5 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120.
Applications
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Western Blot: Do not boil cell lysates prior to SDS-PAGE. Flow Cytometry: 1-4ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CCR5 peptide (Met1-Lys22) KLH conjugate
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD195.
