Goat Anti-CCR11 (Chemokine Receptor 11, CKRB)
Chemokine receptors are specialized proteins that function at the surface of cells, and enable chemokines to trigger internal cellular processes. Chemokines and their receptors play a pivotal role in lymphocyte trafficking, recruiting and recirculation. Chemokines act through seven transmembrane G protein-coupled receptors. The chemokine system is increasingly recognized to play an important role in lymphocyte biology.
CCR11 is a G protein-coupled, seven-transmembrane receptor protein that plays a role in the chemotaxis and trafficking of leukocytes. Showing similar binding affinities as does CCR2 and CCR10, CCR11 has been shown to specifically bind the MCP- (monocyte chemoattractant protein) family of chemokines, including MCP-4, MCP-2, and MCP-1. It was also shown to bind eotaxin. Though CCR11 shares functional similarity to CCR2 because it recognizes members of the MCP family, CCR11 has a distinct expression pattern.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:37,500 Western Blot: 1:500 Immunohistochemistry (Paraffin): 1:125 Optimal dilution determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa4-31, EQNQSTDYYYEENEMNGTYDYSQYELIC, of human CCR11
Form
Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.09% sodium azide
Purity
Purified by affinity chromatography
Specificity
Recognizes human CCR11. Peptide sequence is < 50% identical to other human chemokine receptors in this region. Species Crossreactivity: mouse
