Technical Data

C2102
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-CCR10 (CC Chemokine receptor 10, CC-CCR-10, G protein-coupled receptor 2, GRP2)

Recently, a function has been assigned to a previously identified orphan chemokine receptor, GPR-2. This receptor, also called CCR10, binds with monocyte chemoattractant protein (MCP)-1, MCP-3 and MIP-1 beta. CCR10 is expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells, T cells as well as in skin-derived Langerhans cells. CCR10 may play a role in both skin homeostasis as well as a potential role in inflammatory responses.

Applications
Suitable for use Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml In A-375, ~50kD band is observed. Optimal dilutions to be determined by the researcher.
Positive Control: A-375 or MCF-7
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Peptide corresponding to amino acids 350-362 of human CCR10.
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 0.2% gelatin.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes CCR10. Species Crossreactivity: Human. Predicted to crossreact with mouse. In this region, mouse and human amino acid sequences are 100% identical.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Bonini JA, et al. DNA Cell Biol 16(10): 1249-56 (1997). 2. Bonini JA, et al.. DNA Cell Biol 16(9): 1023-30 (1997). 3. Jarmin DI, et al. J Immunol 164(7): 3460-4 (2000). 4. Homey B, et al.. J Immunol 164(7): 3465-70 (2000). 5. Wang W, et al.. J Biol Chem 275(29): 22313-23 (2000).
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net