MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
CD1 is primarily expressed in cortical thymocytes and dendritic cells in the dermis, afferent lymph, lymph node, spleen and thymus, and also on B lymphocytes and monocytes. This antibody immunoprecipitates antigens at 12kD and 46kD. It is unreactive with granulocytes, platelets and erythrocytes.
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Flow Cytometry: 10ul labels 10e6 cells in 100ul Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Sheep thymocytes.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the sheep CD1 cell surface antigen. Species Crossreactivity: goat and bovine.