C2251-53H
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
BiotinIsotype
IgG1,kClone Number
7H177Grade
Affinity PurifiedApplications
FCCrossreactivity
HuShipping Temp
Blue IceStorage Temp
4°C (-20°C Glycerol)Mouse Anti-CD1a (Langerhans Cells) (Biotin)
The human CD1 genetic locus consists of five closely related genes (1). Three of the gene products (CD1a, CD1b and CD1c) have been officially clustered using antibodies and are expressed on cortical thymocytes, a subset of B cells, and on most professional antigen presenting cells (2). CD1a is an important marker to help identify developmental pathways of dendritic cells (5). CD1 molecules have a specialized lipid antigen-presenting function (3,4) and it is unlikely that they present conventional peptide antigens.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol
1. Five x 10e5 cultured human CEM cells were washed and incubated 45 minutes on ice with 80ul of C2251-53H at 5ug/ml. 2. Cells were washed twice and incubated with S7973-88 Streptavidin (PE). 3. After which they were washed three times, fixed and analyzed using a BD FACS Calibur. 4. Cells stained positive with a mean shift of 1.68 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. 5. Binding was 98% blocked when pre incubated 10 minutes with 20ul of C2251-53G at 0.5 mg/ml.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Human Jurkat tumor cells.
Form
Supplied as a liquid in PBS, pH 7.5,, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD1a at~49kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Leukocyte Typing III (A.J. McMichael, et al, eds.) Oxford University Press, Oxford, (1987) p. 882.2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 337-341. 3. E.M. Beckman, et al, (1994). 4. Nature 372: 691-694. R.S. Blumberg, et al, (1995) Immunol Rev 147: 5-29. 5. T. Ito, et al, (1999) J Immunol 163: 1409-1419.USBio References
No references available