Technical Data

C2252-02Q
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
SN13 (K5-1B8)
Grade
Affinity Purified
Applications
FC IF IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD1b (T-cell Surface Glycoprotein CD1b, CD1b)

CD1b is a 43kD member of the immunoglobulin superfamily also known as R1. It is a type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with B2-microglobulin. In humans, CD1 family consists of group I proteins (CD1a, CD1b, CD1c), group II (CD1d), and group III (CD1e). CD1b plays a role in non-peptide glycolipid antigen presentation to CD1-restricted T cells. It is expressed on cortical double positive and single positive thymocytes, Langerhans cells, and dendritic cells. In addition to antigen presentation, CD1b has been implicated in thymic T cell development.

Applications
Suitable for use in Immunofluorescence, Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤0.5ug/10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Membrane antigen preparation of human thymocytes
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CD1b.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hayes, S.M., et al. 2001. J. Immunol. 166:403. (IF) 2. Martin, L.H., et al. 1987. P. Natl. Acad. Sci. USA 84:918993. (IF) 3. Porcelli, S., et al. 1992. Nature 360:5937. 4. Giuliani, A., et al. 2001. Infect. Immun. 69:746170.
USBio References
No references available
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