C2254-07K
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG2a,kClone Number
HIT3aGrade
Affinity PurifiedApplications
FCCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CMouse Anti-CD3 (Early T Cell Marker, T Cell receptor Complex, TCR)
CD3E is a 20kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3E, one CD3y, one CD3delta, one CD3Z (CD247), and a T-cell receptor (a/B or y/delta) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. This antibody is able to stimulate T cell activation.
Applications
Suitable for use in Flow Cytometry Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤0.25ug/10e5 cells in 100ul Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human CD3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Schlossman, S., et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Knapp, W.1989. Leucocyte Typing IV. Oxford University Press, New York. 3. Barclay, N., et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. 4. Sedelies, K. A., et al. 2004. J. Biol. Chem. 279:26581. 5. Rivollier, A., et al. 2004. Blood 104:4029. 6. Scharschmidt, E., et al. 2004. Mol. Cell Biol. 24:3860. 7. Smeltz, R. B., 2007. J. Immunol. 178:4786. 8. Sheng, W. et al.2007.ELSEVIER.580:6819. 8. Barclay, N., et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 9. Beverly, P., et al. 1981. Eur. J. Immunol. 11:329. 10. Lanier, L., et al. 1986. J. Immunol. 137:2501-2507.USBio References
No references available