C2254-08C
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1,kClone Number
UCHT1Grade
Affinity PurifiedApplications
FC IHCCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CMouse Anti-CD3 (Early T Cell Marker, T Cell receptor Complex, TCR)
CD3E is a 20kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3E, one CD3y, one CD3delta, one CD3Z (CD247), and a T-cell receptor (a/B or y/delta) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. This antibody is able to induce T cell activation.
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: ≤0.25ug/10e5 cells in 100ul Immunohistochemistry (acetone-fixed, frozen): 5-10ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human CD3. Species Crossreactivity: chimpanzee.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Salmeron, A., et al. 1991. J. Immunol. 147:3047. 2. Graves, J., et al. 1991. J. Immunol. 146:2102. 3. Lafont, V., et al. 2000. J. Biol. Chem. 275:19282. 4. Ryschich, E., et al. 2003. Tissue Antigens 62:48. 5. Thompson, A.G., et al. 2004. J. Immunol. 173:1671. 6. Sakkas, L. I., et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. 7. Mack, C.L., et al. 2004. Pediatr. Res. 56:79. 8. Thakral, D., et al. 2008. J. Immunol. 180:7431. (FC) 9. Van Dongen, J.J.M., et al. 1988. Blood 71:603. (WB). 10. Yoshino, N., et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC). 11. Barclay, N., et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 12. Beverly, P., et al. 1981. Eur. J. Immunol. 11:329. 13. Lanier, L., et al. 1986. J. Immunol. 137:2501-2507.USBio References
No references available