Mouse Anti-CD3 (Early T Cell Marker, T Cell receptor Complex, TCR)
CD3 is a complex composed of d, g, e, and z chains. They are 20-25kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation.
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤0.25ug/10e6 cells in 100ul volume Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
F344 rat spleen cells stimulated with PMA and calcium ionophore
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity
Recognizes rat CD3.
