Technical Data

C2255-11DX
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
RFT-4g
Grade
Purified
Applications
FC IHC IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Mouse Anti-CD4 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (Azide Free)

CD4 is a 59kD type I transmembrane glycoprotein and a member of the Ig-SF of cell surface receptors. It is expressed predominantly on the “helper/inducer” subpopulation of mature T lymphocytes, and on monocytes and macrophages. Domains 1 and 2 bind to MHC Class II antigens, while domains 3 and 4 may be involved in cis interactions with the CD3/TCR complex. CD4 functions as an accessary molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.1-6

Applications
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤ 1ug/10e6 cells Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Thymocytes and E-rosetted lymphocytes
Form
Supplied as a liquid in PBS. No preservative added.
Purity
Purified
Specificity
Recognizes human CD4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.|2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD4 Section, Academic Press, New York, p. 141.|3. Parne, J.R. 1984. Adv. Immunol. 44:265.|4. Littman, D.R., ed. 1996. The CD4 Molecule. In: Curr. Top. Microbiol. Immunol. 205.|5. Sakihama, T., A. Smolyar, and E.L. Reinherz. 1995. Immunol. Today 16:581.|6. Vignali, D.A., R.T. Carson, B. Chang, R.S. Mittler, and J.L. Strominger. 1996. J. Exp. Med. 183:2097.
USBio References
No references available
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