Technical Data

C2256-12K
Clone Type
Monoclonal
Host
Mouse
Source
Rat
Isotype
IgG1
Clone Number
OX-19
Grade
Affinity Purified
Applications
FC IHC IP
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD5 (CD5 Antigen, CD5 Molecule, LEU1, Lymphocyte Antigen CD5, Lymphocyte Antigen T1/Leu 1, Lymphocyte Glycoprotein T1/Leu1, p56 62, T cell Surface Glycoprotein CD5, T1)

The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained (1). Anti-CD5 also reacts with a distinct subset normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:100; 10ul labels 10e6 cells in 100ul Immunohistochemistry (paraffin): PLP fixation is recommended. Optimal dilutions to be determined by the researcher.
Hybridoma
NS1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Rat thymocyte glycoproteins.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes rat CD5.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Dallman, M.J. et al. (1982) The roles of host and donor cells in the rejection of skin allografts by T cell-deprived rats injected with syngeneic T cells. Eur. J. Immunol. 12: 511-518, 2. Dallman, M.J. et al. (1984) MRC OX-19: A monoclonal antibody that labels rat T lymphocytes and augments in vitro proliferative responses. Eur. J. Immunol. 14: 260-267, 3. Huitinga, I. et al. (1995) Macrophages in T cell line-mediated, demyelinating, and chronic relapsing experimental autoimmune encephalomyelitis in Lewis rats. Clin. Exp. Immunol. 100: 344-351, 4. Whiteland, J.L. et al. (1995) Immunohistochemical Detection of T-Cell Subsets and other Leucocytes in Paraffin-embedded Rat and Mouse Tissues with Monoclonal Antibodies. J. Histochem. Cytochem. 43: 313-320.
USBio References
No references available
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