C2256-25L
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1,kClone Number
UCHT2Grade
Affinity PurifiedApplications
FC IHC WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CMouse Anti-CD5 (T-cell Surface Glycoprotein CD5, Lymphocyte Antigen T1/Leu-1, LEU1)
CD5 is a 67kD single chain type I glycoprotein also known as Leu-1, Ly-1 and T1. It is a member of the scavenger receptor superfamily found on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-Cells), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions upon binding to ligands such as CD72.
Applications
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤1ug/10e6 cells in 100ul Immunohistochemistry: acetone-fixed frozen Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CD5. Species Crossreactivity: chimpanzee.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Knapp, W., et al. 1989. Leucocyte Typing IV, Oxford University Press. New York. 2. Renaudineau, Y., et al. 2005. Blood 106:2781. (WB, IHC) 3. Porter, J.C. and Hogg, N., 1997. J. Cell Biol. 138:1437. 4. Saliba, A.E., et al. 2010. P. Natl. Acad. Sci. USA 107:14524. 5. Kap, Y., et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC) 6. Kipps, T. 1988. Adv. Immunol. 47:117. 7. Resnick, D., et al. 1993. Trends Biochem. Sci. 19:5. 8. Wood, G.S., et al. 1992. Am. J. Pathol. 14:789.USBio References
No references available