Rabbit Anti-CD8A, CT (T-cell Surface Glycoprotein CD8 alpha Chain, T-lymphocyte Differentiation Antigen T8/Leu-2, MAL)
CD8a is a 32-34kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the a3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Formalin fixed paraffin embedded): 1:25-1:100 Immunohistochemistry (Frozen): 1:100-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human CD8 at C-terminal conjugated to KLH.
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 20mg/ml BSA, 0.05% sodium azide.
Specificity
Recognizes human CD8.
