Technical Data

C2261-40C
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
Biotin
Isotype
IgG1,k
Clone Number
7H202
Grade
Affinity Purified
Applications
FC WB
Crossreactivity
Hu Ro
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral Endopeptidase 24.11, EC3.4.24.11) (Biotin)

Human CD10 is a well known marker for common acute lymphoblastic leukemia antigen (CALLA). CD10 is expressed on normal early B cells in bone marrow and is also on fetal thymocytes. CD10 expression is lost as the T and B lineage cells mature and only appears again on activated germinal center B cells. CD10 is present on neutrophils. CD10 is a neutral endopeptidase and hydrolyzes peptide bonds on the amino side of hydrophobic amino acids.

Applications
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Membrane preparation of human B-lineage leukemia cells.
Form
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride,150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Purity
Purified by Protein A affinity chromatography. ~ 95% (SDS-PAGE)
Specificity
Recognizes human membrane-associated peptidase CD10 at ~100kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. H. Matsuzaki, et al, (1987) Cancer Research 47: 2160-2166. |2. W.C. Biddle, et al, (1989) Leukemia Research 13: 699-707. |3. H. Hara, et al, (1988) Cancer Research 48: 4673-4680. |4. Leukocyte Typing V (S.F. Schlossman,et al, eds.) Oxford University Press, Oxford, (1995) p. 505-507.
USBio References
No references available
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