Mouse Anti-CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral Endopeptidase 24.11, EC3.4.24.11)
The human CD10 cell surface antigen is a 100kD glycoprotein also known as CALLA (common acute lymphoblastic leukemia antigen). CD10 is expressed on a variety of cell types, including immature and germinal center B cells, lymphoblastic leukemia cells and also on the brush border of kidney epithelial cells.
Applications
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (Frozen, paraffin): 1:80. Requires antigen retrieval using heat treatment with sodium citrate buffer pH 6.0 prior to staining paraffin sections. Western Blot: 1:50-1:100 Optimal dilutions to be determined by researcher.
Immunohistochemistry Positive Controls
Tonsil, intestine, kidney
Hybridoma
p3-NS1-Ag4-1 myeloma cells with spleen cells from mice.
Recommended Secondary Antibodies
I1904-04D: IgG, H&L (AP) Pab Gt xMo I1904-06F: IgG, H&L (FITC) Pab Gt xMo I1904-06C: IgG, H&L (HRP) Pab Gt xMo I1903-07E: IgG, (HRP) Pab Rb xMo I1903-13A: IgG, (PE) Pab Gt xMo
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human CD10.
Form
Supplied as a liquid in 0.09% sodium azide.
Specificity
Recognizes human CD10.
