Mouse Anti-CD11a (Leukocyte Function Associated Antigen, LFA-1, Integrin aL) (Biotin)
Human CD11a (alpha L integrin) complexes with CD18 (beta2 integrin) to form the LFA-1 heterodimer adhesion molecule which binds to three ICAM's 1, 2, and 3, also designated CD54, CD102, and CD50 respectively. LFA-1 is expressed on lymphocytes, monocytes and neutrophils and plays a role in cell-cell adhesion.
Functional Application: Antibody blocks binding of ICAM-1 and ICAM-3 to LFA-1 at 5-10ug/ml (7).
Applications
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a liquid in PBS, pH 7.2, 5% glycerol, 0.2% BSA, 0.05% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the I domain of the integrin alpha L subunit (CD11a) of about 180kD.
