Technical Data

C2262-27E
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Conjugate
PE
Isotype
IgG2a,k
Clone Number
I21/7
Grade
Purified
Applications
FC IHC IP WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Rat Anti-CD11a (Leukocyte Function Associated Antigen, LFA-1, Integrin aL) (PE)

Leukocyte Function-associated Antigen-1 (LFA-1) is a member of the integrin family of cell adhesion molecules (1, 2). The antigen is expressed on thymocytes, T and B lymphocytes, and on non-lymphoid cells in the bone marrow (2). CD11a appears to be an essential component for T-cell activation by antigen-presenting cells and is important for cell-mediated killing. Co-stimulatory signaling by LFA-1 is achieved by pairing with its ligand, CD54/ICAM-1 (1, 2).

Applications
Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤0.2ug/10e6 cells Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Whole BW5147 cells
Form
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE).
Purity
Purified
Specificity
Recognizes the alpha subunit (Mr 180kD) of LFA-1. Species Crossreactivity: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Altin, J.G., E.B. Pagler and C.R. Parrish. 1994. Eur. J. Immunol. 24:450.|2. Springer, T.A., D. Davignon, M. Ho, K. Kurzinger, E. Martz, and F. Sanchez-Madrid. 1982. Immunol. Rev. 68:171.|3. Makino, M., K. Yoshimatsu, M. Azuma, Y. Okada, Y. Hitoshi, H. Yagita, K. Takatsu, and K. Komuro. 1995. J. Immunol. 155:974.|4. Connolly, M.K., E.A. Kitchens, B. Chan, P. Jardieu, and D. Wofsy. 1994. Clin. Immunol. Immunopath. 72:198.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net