Technical Data

C2262-36-ML550
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™550
Isotype
IgG1,k
Clone Number
ICRF44
Grade
Purified
Applications
FC IHC
Crossreactivity
Hu Mk Po
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-CD11B (Integrin alpha-M, CD11 Antigen-like Family Member B, CR-3 alpha Chain, Cell Surface Glycoprotein MAC-1 Subunit alpha, Leukocyte Adhesion Receptor MO1, Neutrophil Adherence Receptor, ITGAM, CR3A, MGC117044) (MaxLight 550)

MaxLight™ 550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 ( alphaMBeta2) molecule, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3). ITGAM is also known as CR3A, and cluster of differentiation molecule 11B (CD11B).
Applications
Suitable for use in Immunohistochemistry, Flow Cytometry and Blocking Studies. Other applications not tested.
Recommended Dilution
Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Rheumatoid synovial cells and human monocytes
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Purity
Purified
Specificity
Recognizes human CD11b. Species Crossreactivity: Porcine, Rhesus. Cynomolgus, Chimpanzee and Baboon

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.|2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.|3. Springer, T.A. 1994. Cell 76:301.|4. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD11b Section, Academic Press, New York, p. 158.|5. Lee, J.O., L.A. Bankston, M.A. Arnaout, and R.C. Liddington. 1995. Structure 3:1333.|6. Zhang, L.., and E.F. Plow. 1996. J. Biol. Chem. 271:18211.|7. Davis, G.E. 1992. Exp. Cell. Res. 200:242.|8. Perry, H.R., and R.F. Todd, III. 1996. Immunol. Today 17:209.
USBio References
No references available
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