Technical Data

C2262-51V
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
CBRM1/5
Grade
Affinity Purified
Applications
FC IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD11b, Active (Integrin alpha-M, CD11 Antigen-like family Member B, CR-3 alpha Chain, Cell Surface Glycoprotein MAC-1 Subunit alpha, Leukocyte Adhesion Receptor MO1, Neutrophil Adherence Receptor, ITGAM, CR3A)

The CBRM1/5 antibody reacts with an activated form of human CD11b, a 165-170kD type I transmembrane glycoprotein also known as aM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin B2 (CD18) and is expressed on granulocytes, monocytes/macrophages dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils, as well as adhesion, phagocytosis and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and Factor X. The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. The epitope recognized by CBRM1/5 is contained in the I domain of the a chain. This antibody blocks cell adhesion to fibrinogen and ICAM-1.

Applications
Suitable for use in Immunoprecipitation, Flow Cytometry and Blocking of the Binding of ICAM-1 and Fibrinogen. Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤2ug/10e5 cells in 100ul Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes the activated form of human CD11b. Does not recognize "non-activated" CD11b.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Diamond, M., et al. 1993. J. Cell Biol. 120:545. 2. Bryn, T., et al. 2006. J. Immunol. 176: 7361.J Immunol 3. Sithu, S.D., et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200. 4. Barthel, SR.,et al.2006. Am J. Respir Cell Mol Biol.35:378. 5. Pillay, J.,et al. 2010. J. Leukoc Biol. 88:211. 6. Stewart, M., et al. 1995. Curr. Opin. Cell Biol. 7:690. 7. Petty, H., et al. 1996. Immunol. Today 17:209.
USBio References
No references available
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