Suitable for use in Flow Cytometry. Other applications not tested.

Flow Cytometry: 5x10e5 ficoll prepared human peripheral blood mononuclear cells per tube were washed and pre-incubated 5 min with 20ul of 250ug/ml human IgG (To block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD11c antibody at 10ug/ml. Cells were washed twice and incubated with 50ul of Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A 15% sub population of cells stained positive with a mean shift of 1.01 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human rheumatoid synovial fluid cells/monocytes

Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 100 mM potassium chloride, 150mM sodium chloride. No preservative added.

Purified by Protein A affinity chromatography.

Recognizes human CD11c.