Technical Data

C2262-96-ML490
Clone Type
Monoclonal
Host
Mouse
Source
Porcine
Conjugate
MaxLight™490
Isotype
IgG1
Clone Number
2F4/11
Grade
Affinity Purified
Applications
FC IHC IP WB
Crossreactivity
Po
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-CD11R3 (MaxLight 490)

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Westen Blot. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Positive Control for Immunohistochemistry
Porcine spleen
Hybridoma
X63-Ag.8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Porcine alveolar macrophages.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes porcine CD11R3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Bullido, R. et al. (1996) Monoclonal antibody 2F4/11 recognizes the alpha chain of a porcine beta 2 integrin involved in adhesion and complement mediated phagocytosis. J. Immunol. Methods 195: 125-134, 2. Domingues, J. et al. (2001) Workshop studies on monoclonal antibodies in the myeloid panel with CD11 specificity. Vet. Immunol. Immunopathol. 80: 111-119, 3. Sanchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/ macrophages correlates with permissiveness to African swine fever infection. Arch. Virol. 148: 2307-2323, 4. Van de Walle, G. et al. (2003) Transmission of pseudorabies virus from immune-masked blood monocytes to endothelial cells. J. Gen. Virol. 84: 629-637, 5. Alvarez, B. et al. (2000) Molecular and functional characterization of porcine LFA-1 using monoclonal antibodies to CD11a and CD18. Xenotransplantation 7: 258-266
USBio References
No references available
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