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C2262-96-ML490 Mouse Anti-CD11R3 (MaxLight 490)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Porcine
Conjugate
MaxLight™490
Isotype
IgG1
Clone Number
2F4/11
Grade
Affinity Purified
Applications
FC IHC IP WB
Crossreactivity
Po
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Westen Blot. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Positive Control for Immunohistochemistry
Porcine spleen
Hybridoma
X63-Ag.8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Porcine alveolar macrophages.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes porcine CD11R3.
References
1. Bullido, R. et al. (1996) Monoclonal antibody 2F4/11 recognizes the alpha chain of a porcine beta 2 integrin involved in adhesion and complement mediated phagocytosis. J. Immunol. Methods 195: 125-134, 2. Domingues, J. et al. (2001) Workshop studies on monoclonal antibodies in the myeloid panel with CD11 specificity. Vet. Immunol. Immunopathol. 80: 111-119, 3. Sanchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/ macrophages correlates with permissiveness to African swine fever infection. Arch. Virol. 148: 2307-2323, 4. Van de Walle, G. et al. (2003) Transmission of pseudorabies virus from immune-masked blood monocytes to endothelial cells. J. Gen. Virol. 84: 629-637, 5. Alvarez, B. et al. (2000) Molecular and functional characterization of porcine LFA-1 using monoclonal antibodies to CD11a and CD18. Xenotransplantation 7: 258-266
USBio References
No references available
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