C2264-01P
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
PEIsotype
IgG1Clone Number
Tuk 1Grade
Highly PurifiedApplications
FCCrossreactivity
HuShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeMouse Anti-CD13 (Aminopeptidase N, APN, AP-N, hAPN, Alanyl Aminopeptidase, Alanyl (Membrane) Aminopeptidase, ANPEP, Microsomal Aminopeptidase, Aminopeptidase M, AP-M, gp150, LAP1, Myeloid Plasma Membrane Glycoprotein CD13, P150, PEPN) (PE)
EC=3.4.11.2
CD13 is expressed on cells of the myelomonocyte lineage including myeolomonocyte precursors, monocytes, basophils, eosinophils, neutrophils and myeloid leukaemias. The antigen is also present on small intestine and renal proximal tubule epithelial cells, synaptic membranes of the central nervous system, fibroblasts and osteoclasts.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 5ul/10e6 cells. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Form
Supplied as a liquid in PBS, BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity
Recognizes human CD13 monocytes and granulocytes. Other reactive cells in flow cytometry incl. myeloid leukemias and myeloid cell lines. Other reactive cells in immunohistology incl. histiocytes, high endothelial venules and kidney tubules.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Griffin, J.D., et al., 1981, J. clin. Invest. 68, 932. 2. Look, A.T., et al., 1985, J. clin. Invest. 75, 569. 3. Sakai, K., et al., 1987, Cancer Res. 47, 5572. 4. Look, A.T., et al., 1989, J. clin. Invest. 83, 1299.USBio References
No references available