Technical Data

C2265-33T
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
PE
Isotype
IgG2a
Clone Number
7H221
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Mouse Anti-CD14 (PE)

Human CD14 is strongly expressed on myelomonocyte lineage cells including monocytes, macrophages, and a subset of granulocytes. CD14 is a high affinity receptor for complexes of lipopolysaccharide and lipopolysaccharide binding protein. A soluble form of CD14 also binds to LPS and LBP.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
Immunogen
Human thymocytes/Sezary T cells
Form
Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.05% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes a membrane bond CD14 molecule of about 53kD. Antibody will also immunoprecipitate a soluble form of CD14.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. N. Hogg, et al, (1984) Immunology 53: 753-767. |2. M.O. Labeta, et al, (1993) Eur J Immunol 23: 2144-2151. |3. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) |4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 778-788. |5. S.D. Wright, (1995) J Immunol 155: 6-8.
USBio References
No references available
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