Technical Data

C2265-33U
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2a
Clone Number
3H493 (UCHM1)
Grade
Affinity Purified
Applications
FC IF IHC IP
Crossreactivity
Hu Mk
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD14 (CD14 Antigen, Lipopolysaccharide Receptor, LPSR, Monocyte Differentiation Antigen 14, Myeloid Cell Specific Leucine Rich Glycoprotein)
CD14 antigen, Lipopolysaccharide receptor, LPSR, Monocyte Differentiation Antigen 14, Myeloid cell specific leucine rich glycoprotein

CD14 is found chiefly on monocytes. UCHM1 was clustered at the Third International Workshop and Conference on Human Leucocyte Differentiation Antigens, Oxford 1986, as CDw14, group A.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:50. 10ul labels 10e6 cells in 100ul Immunohistochemistry (Frozen): The epitope recognized by this antibody is sensitive to formaldehyde fixation and tissue processing. Acetone fixation for frozen sections is recommended. Not suitable for use with paraffin sections. Optimal dilutions to be determined by the researcher.
Hybridoma
NS1-Ag4/1myeloma cells with human thymocytes and PBLs
Immunohistochemistry Positive Control
Tonsil
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human Thymocytes followed by peripheral blood mononuclear cells.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD14. Recognizes a monocyte surface antigen of 55kD found chiefly on monocytes. Gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells in tonsil tissue sections. Skin Langerhans cells are always negative. Also reacts with Kupffer cells and sinus lining cells on the liver. |Species Crossreactivity: Cynomolgus monkey, Rhesus monkey, fish, trout.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hogg, N. et al. (1984) Monoclonal antibodies specific for human monocytes, granulocytes and endothelium. Immunol. 53: 753-768, 2. Linch, D.C. et al. (1984) Monoclonal antibodies differentiation between monocyte and non-monocytic varients of AML. Blood. 63: 566-573, 3. Hogg, N. and Horton, M.A. (1987) Myeloid antigens: new and previously defined clusters in Leucocyte Typing III White Cell differentiation antigens. Edited by McMichael, A.J., Beverley, P.C.L., Cobbold, S., Crumpton, M.J., Gilks, W., Gotch, F.M., Hogg, N., Horton, M., Ling, N., Mackenna, I.C.M., Mason, D.Y., Milstein, G., Speghelhalter., D. and Waldmann, H. et al, 4. Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocytes. Leucocyte Typing IV. Oxford University Press p 1058-1063, 5. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49 (2): 97-110. 6. Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands. J. Immunol. 176: 5730-5734.7. Bournazos, S. et al. (2008) Monocyte functional responsiveness after PSGL-1-mediated platelet adhesion is dependent on platelet activation status. Arterioscler Thromb Vasc Biol. 28: 1491-8 8. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 12:2. 9. Hsu, T.L. et al. (2002) Modulation of dendritic cell differentiation and maturation by decoy receptor 3. J Immunol. 168: 4846-53. 10. Karlsson, H. et al. (2002) Innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora. Infect Immun. 70: 6688-96.
USBio References
No references available
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