Technical Data

C2266-18E
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgM,k
Clone Number
HI98
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD15 (Lewis x, Le x, Hematopoietic Differentiation Antigen, SSEA-1, Stage-specific Embryonic Antigen, X Hapten, 3-fucosyl-N-acetyllactosamine, 3-FAL)

CD15 is a Lewis blood group related carbohydrate antigen known as X-hapten, Lewis X, 3-FAL, 3-fucosyl-N-acetyllactosamine, or CD15.The expression pattern of SSEA-1 antigen is different from human and mouse. In mouse, SSEA-1 is expressed on embryonic stem cells (ES), embryonal carcinoma cells (EC), 8-cell to blastocyst embryos, and a subset of embryonic inner cell mass. The expression on murine ES cells is decreased upon differentiation. In human, however, SSEA-1 is not found on undifferentiated ES cells, but its expression is upregulated along with differentiation. CD15 is highly expressed on adult human granulocytes. It has been reported that SSEA-1 plays a role in cell adhesion and regulation of cell differentiation.

Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: frozen and paraffin Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human CD15. Species Crossreactivity: chimpanzee.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Knapp, W., et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Schlossman, S., et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 3. McMichael, A., et al. 1987. Leucocyte Typing III. Oxford University Press. New York. 4. Yoshino, N., et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 1. Stocks, S.C., et al. 1990. Biochem. J. 268:275.
USBio References
No references available
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