Mouse Anti-CD15 (Alpha-(1,3)-fucosyltransferase, ELAM-1 Ligand Fucosyltransferase, ELFT, FCT3A, Fucosyltransferase 4, FUT4, Fucosyltransferase IV, Fuc-TIV, FucT-IV, FUTIV, Galactoside 3-L-fucosyltransferase, LeX, Stage Specific Embryonic Antigen 1, SSEA 1, SSEA-1)
Fucosyltransferase 4 (alpha (1,3) Fucosyltransferase, Myeloid-specific), EC=2.4.1.-
CD15 is otherwise known as Lewis X and stage-specfic embryonic antigen-1 (SSEA-1). The CD15 antigen is itself a terminal trisaccharide, Gal-beta1-4 (Fuc-alpha1-3) GlcNAc (3-FAL), which is found on a variety of glycoproteins and glycolipids. CD15 is predominately expressed by peripheral blood granulocytes and various epithelial tissues. It plays an important role in cell adhesion and has been used as a marker for diagnosis of Hodgkin’s lymphoma.
Applications
Suitable for use in Flow Cytometry. Other applications have not been tested.
Recommended Dilutions
Flow Cytometry: Neat. Use 20ul to label 1x10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide.
Specificity
Recognizes human CD15.
