Mouse Anti-CD16 (Fc gamma RIII alpha, NK cells)
CD16 is a 50-65kD cell surface molecule, which is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes. In addition, CD16B exists as two allelic variants NA1 and NA2. This antibody recognises all polymorphonuclear cells irrespective on their NA phenotype.
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer, pH 6.0 is recommended for this purpose. Immunohistochemistry: Frozen sections Flow Cytometry: 1:50 -1:200; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Immunohistochemistry Positive Control
Human tonsil
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD16. Recognizes an epitope in the first membrane-distal domain of CD16 and reacts with both CD16a and CD16b. Crossreacts with CD16 from rhesus macaques, Macaca mulatta.
