Technical Data

C2268-01G
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Conjugate
PE/Cy7
Isotype
IgG2a
Clone Number
93
Grade
Purified
Applications
FC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-CD16,32 (PE/Cy7)

The lymphocyte Fc-gamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta.1, 2 The Fc-g type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios.1 The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.4 Besides identifying Fc-gammaR+ cells, monoclonal antibodies to the Fc-gII/III receptor have been used to block Fc receptor binding of IgG, Fc-mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc-gR for parasitic invasion.4-6

Applications
Suitable for use in Flow Cytometry and blocking of Fc gamma receptors. Other applications not tested.
Recommended Dilutions
Flow Cytometry: ≤0.2ug/10e6 cells Optimal dilutions to be determined by the researcher..
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt at 4°C. Freezing PE/CY7 conjugates will result in a substantial loss of activity. PE/CY7 conjugates are sensitive to light.
Immunogen
VH81X Tg B cells and T220 stromal cell line
Form
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/Cy7.
Purity
Purified
Specificity
Recognizes murine CD16/32 (CD16/Fc-gammaII and CD32/Fc-gIII receptors). Recognizes a conformational epitope formed by Fc-gII and Fc-gIII receptors.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Unkeless, J. C., et al. 1995. Semin. Immunol. 7:37.|2. Warmerdam, P.A., et al. 1992. Immunobiol. 185:175.|3. Huizinga, T. W., et al. 1989. J. Immunol. 142:2365.|4. Ho, A. S-Y., et al. 1995. Mol. and Cell. Biol. 15:5043.|5. John Kearney, personal communication.|6. Kurlander, R.J., D. M. Ellison and J. Hall. 1984. J. Immunol. 133:855.|7. Araujo-Jorge, T., et al. 1993. Infection and Immunity 61:4925.|8. Titus, J. A., et al. 1984. J. Immunol. 133:556.
USBio References
No references available
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