C2269-11B
Clone Type
MonoclonalHost
RatSource
MouseConjugate
FITCIsotype
IgG2a,kClone Number
C71/16Grade
PurifiedApplications
FC IHC IP WBCrossreactivity
MoShipping Temp
Blue IceStorage Temp
-20°CRat Anti-CD18 (Leukocyte Function Associated Antigen, LFA-1 beta2) (FITC)
CD18 represents the common beta2 integrin subunit that associates non-covalently with the alpha beta chains of CD11a/LFA-1a, CD11b/Mac-1, and CD11c to form various integrin heterodimers. It is expressed strongly on lymphocytes and monocytes, and weakly on granulocytes, CD18 mediates a variety of heterotypic and homotypic intercellular adhesion reactions, and it regulates the ligand-binding activities of the various CD11/CD18 complexes. The antibody has not been reported to inhibit cell adhesion.1-4
Applications
• Identification and enumeration of CD18+ cells by flow cytometry 1 • Immunohistochemistry (paraffin embedded and frozen sections) 1 • Immunoprecipitation 1 • Western blotting 1
Recommended Dilution
Flow Cytometry: ≤ 1ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Cell membrane lysates derived from mouse T cell lymphoma BW5147
Form
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with FITC.
Specificity
Recognizes common beta2 subunit (Mr 95kD) of the LFA-1 integrin complex.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Trowbridge, I. and M.B. Omary, 1981. J. Exp. Med. 154:1517.|2. Mazzone, A. and G. Ricevuti. Haemotoligica, 80:161.|3. Lub, M., Y. van Kooyk, and C. G. Figdor, 1995. Immunol. Today. 16:479.|4. Springer, T.A., G. Galfre, D.S. Secher, and C. Milstein. 1978. Eur. J. Immunol. 8:539.USBio References
No references available