C2269-50R
Clone Type
MonoclonalHost
RatSource
MouseConjugate
PE/Cy5.5Isotype
IgG2a,kClone Number
6D5Grade
PurifiedApplications
FC IPCrossreactivity
MoShipping Temp
Blue IceStorage Temp
-20°CRat Anti-CD19 (B Lymphocyte Antigen CD19, B4, Differentiation Antigen CD19, Leu 12, Leu-12, MGC12802) (PE/Cy5.5)
CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages.1-7 Terminally differentiated plasma cells do not express CD19.1 In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation.3, 4 All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites.1, 7 Recent studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow.7 Monoclonal antibody 3H1069 recognizes the same epitope as the published clone, 1D3 (1).
Applications
• Flow cytometry 1-3 • Immunoprecipitation 1 • In vivo and in vitro functional studies 1, 2
Recommended Dilution
Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Form
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/Cy5.5.
Specificity
Recognizes CD19 B-cell differentiation antigen, Mr 95kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Krop, I., A.R. Fougerolles, R.R. Hardy, M. Allison, M.S. Schlissel, and D.T. Fearon. 1996. Eur. J. Immunol;. 26:238.|2. Krop, I., A.L. Shaffer, D.T. Fearon, and M.S. Schlissel. 1996. J. Immunol. 157:48.|3. Fearon, D.T. 1993. Curr. Opin. in Immunol. 5:341.|4. Tedder, T.F., L.-J. Zhou, and P. Engel. 1994. Immunol. Today 15:437.|5. Rickert, R.C., K. Rajewsky, and J. Roes. 1995. Nature 376:352.|6. Engel, P., L.-J. Zhou, D.C. Ord, S. Sato, B. Koller, and T.F. Tedder. 1995. Immunity 3:39.|7. Rolink, A., E. ten Boekel, F. Melchers, D.T. Fearon, I. Krop, and J. Andersson. 1996. J. Exp. Med. 183:187.USBio References
No references available