C2269-73
Clone Type
MonoclonalHost
MouseSource
MouseIsotype
IgA,kClone Number
MB19-1Grade
PurifiedApplications
FC IPCrossreactivity
MoShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Mouse Anti-CD19
CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e., B220+/CD43+/HSA+) through fully differentiated B cell stages.1-7 Terminally differentiated plasma cells do not express CD19.1 In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation.3, 4 All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites.1, 7 Recent studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow.7
Applications
• Flow cytometry 1-3 • Immunoprecipitation 1
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
CD19+ mouse pre-B cell line, 300.191
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Specificity
Recognizes CD19 B cell differentiation antigen, Mr 95kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Sato, S., N. Ono, D.A. Steeber, D.S. Pisetsky, and T.F. Tedder. 1996. J. Immunol. 157:4371-4378.|2. Krop, I., A.R. Fougerolles, R.R. Hardy, M. Allison, M.S. Schlissel, and D.T. Fearon. 1996. Eur. J. Immunol. 26:238.|3. Krop, I., A.L. Shaffer, D.T. Fearon, and M.S. Schlissel. 1996. J. Immunol. 157:48.|4. Fearon, D.T. 1993. Curr. Opin. in Immunol. 5:341.|5. Tedder, T.F., L.-J. Zhou, and P. Engel. 1994. Immunol. Today 15:437.|6. Rickert, R.C., K. Rajewsky, and J. Roes. 1995. Nature 376:352.|7. Engel, P., L.-J. Zhou, D.C. Ord, S. Sato, B. Koller, and T.F. Tedder. 1995. Immunity 3:39.|8. Rolink, A., E. ten Boekel, F. Melchers, D.T. Fearon, I. Krop, and J. Andersson. 1996. J. Exp. Med. 183:187.USBio References
No references available