Mouse Anti-CD19 (CD19 Antigen, B Lymphocyte Antigen CD19, B Lymphocyte Surface Antigen B4, B4, Differentiation Antigen CD19, Leu 12, Leu-12, MGC12802)
CD19 Molecule
CD19, a 95kD cell surface glycoprotein, which is expressed by cells of the B cell lineage and follicular dendritic cells. CD19 is absent on plasma cells. CD19 is an important signal transduction molecule which is involved in the regulation of B lymphocyte development, activation and differentiation.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation and Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required Immunohistochemistry (Paraffin): 1:100-1:200. Requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Western Blot: Detetcts a band at ~95kDa in Raji cell lysates under reducing conditions Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo
Hybridoma
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months aafter receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide corresponding to aa539-556 from human CD19, CGPDPAWGGGGRMGTWSTR (C-terminal), conjugated to KLH.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the C-terminal cytoplasmic tail sequence, CGPDPAWGGGGRMGTWSTR of human CD19.
