Technical Data

C2270-02A-ML650
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™650
Isotype
IgG2b
Clone Number
2H7
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
Hu Mk
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-CD20 (CD20 Antigen, B1, B-lymphocyte Cell-surface Antigen B1, Bp35, CD20 Receptor, LEU-16, MGC3969, Membrane-spanning 4-domains Subfamily A Member 1, MS4A1, MS4A2, S7) (MaxLight 650)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 10ul labels 10e6 cells in 100ul Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD20. Species Crossreactivity: Rhesus Monkey.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hultin, L.E. et al. (1993) CD20 (pan B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytes. Cytometry 14: 196, 2. Lifson, J. et al. (2001) Role of CD8+ lymphocytes in control of simian Immunodefiency virus infection and resistance to rechallenge after transient early antiretroviral treatment. J. Virol. 75 (21): 10187 - 10199.
USBio References
No references available
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