C2270-05A
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG2a,kClone Number
L26Grade
PurifiedApplications
IHCCrossreactivity
HuGene ID
931Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-CD20
B-lymphocyte antigen CD20, MS4A1, Membrane-spanning 4-domains subfamily A member 1
B-lymphocyte antigen CD20 (also known as MS4A1; Membrane-spanning 4-domains subfamily A member 1) is a cell surface phosphoprotein involved in the regulation of B cell activation and proliferation (1,2). It is commonly used as a marker to identify B cells and is expressed throughout B cell development, up until their differentiation into plasma cells. CD20 has no known ligand, and its expression and function are largely conserved between human and mouse (1-3). Evidence suggests that CD20 is necessary for store operated calcium (SOC) entry, which leads to elevated cytoplasmic calcium levels required for B cell activation (4,5). Anti-CD20 antibody immunotherapy depletes B cells by activation of the innate monocytic network and is a common treatment for B cell lymphomas, leukemias, and autoimmune diseases (6).
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (paraffin): 1:4000 - 1:16,000 Optimal working dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human tonsillar B cells.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity
Rcognizes endogenous levels of total human CD20
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Stashenko, P. et al. (1980) J Immunol 125, 1678-85.|2. Tedder, T.F. et al. (1985) J Immunol 135, 973-9.|3. Tedder, T.F. et al. (1988) J Immunol 141, 4388-94.|4. Bubien, J.K. et al. (1993) J Cell Biol 121, 1121-32.|5. Li, H. et al. (2003) J Biol Chem 278, 42427-34.|6. Uchida, J. et al. (2004) J Exp Med 199, 1659-69.USBio References
No references available