C2272-66
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG2aClone Number
0.N.89Grade
SupernatantApplications
IHCCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CMouse Anti-CD21 (CR2, C3d Receptor, EBV Receptor)
CD21 is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (Formalin/paraffin): 1:10-1:20 for 60 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes., followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein encoding the external domain of human CD21. Cellular Localization: Cell membrane.
Form
Supplied as a liquid in 0.09% sodium azide.
Specificity
Recognizes human CD21 at ~140kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Schlossman SF et al. eds Leukocyte Typing V, p516-522, Oxford University Press, Oxford, 1995. 2. Aubry JP et al. CD23 interacts with a new epitope on CD21. In Schlossman SF et al eds. Leukocyte Typing V, p535-536, Oxford University Press, Oxford, 1995. 3. Carel JC et al. Structural requirements for C3d,g/Epstein-Barr virus receptor (CR2/CD21) ligand binding, internalization, and viral infection. J Biol Chem 1990, 265(21):12293-12299. 4. Cooper NR et al. Immunobiology of CR2, the B lymphocyte receptor for Epstein-Barr virus and the C3d complement fragment. Annu Rev Immunol 1988, 6:85-113.USBio References
No references available