C2273-91AX
Clone Type
MonoclonalHost
RatSource
MouseIsotype
IgG2a,kClone Number
2G8Grade
PurifiedApplications
FC IPCrossreactivity
MoShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Rat Anti-CD23 (IgE Receptor gp45) (Azide Free)
The CD23 antigen is the low affinity IgE Fc receptor, which is a 49kD protein with 38 and 28kD fragments.1 It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages and platelets.1-6 It is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation.3 The regulation of CD23 surface expression appears to be integral with the complex IgE system, which involves interactions of cells, cytokines, antibodies and regulatory factors.2-6 CD23 has been described as a “membrane bound cytokine," in that the soluble cleavage products of CD23 are themselves able to act as cytokines in vitro.6
Applications
• Identification and enumeration of CD23+ cells by flow cytometry 2, 5 • Differentiation of B cell subpopulations by cell sorting 7 • Immunoprecipitation of CD23 1
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
FcεR isolated from B cell lymphoma cell line M12.4.5
Form
Supplied as a liquid in PBS. No preservative added.
Specificity
Recognizes CD23 low affinity IgE Fc receptor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Rao, M., W. Lee, and D. Conrad. 1987. J. Immunol. 138:1845.|2. Cong Y.Z., Rabin E. and Wortis H.H. 1991. International Immunology. 3:467.|3. Conrad DH. 1991. Monographs in Allergy. 29:9.|4. Vercelli D.and Geha RS. 1989. Annals of Allergy. 63:4.|5. Yu, P., Kosco-Vilbios, M., Richards, M., Kohler, G. and Lamers, M.C. 1994. Nature 369:753.|6. Bonnefoy, J.-Y., Lecoanet-Henchoz, S., Aubry, J.P., Gauchat, J.F. and Graber, P. 1995. Current Opinions in Immunol. 7:355.USBio References
No references available