C2275-89A
Clone Type
MonoclonalHost
RatSource
MouseIsotype
IgM,kClone Number
7D4Grade
PurifiedApplications
FC IPCrossreactivity
MoShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Rat Anti-CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor)
The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail.2 CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested.1, 3, 4 It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells.5, 6 CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus.6, 7 While the antibody does not block the binding of IL-2 to CD25, when used in combination with C2275-82 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.3, 8, 9
Applications
• Flow cytometry 1, 3, 4, 7, 8 • Immunoprecipitation 1, 3
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Specificity
Recognizes low affinity alpha chain IL-2 receptor (IL-2Ra), Mr 55kD. Reacts with an epitope between amino acids 125 and 212.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Malek, T. R., R. J. Robb, and E. M. Shevach. 1983. Proc. Natl. Acad. Sci. U. S. A. 80:5694.|2. Leonard, W.J., et al. 1984. Nature 311:626.|3. Ortega, G., R. J. Robb, E. M. Shevach, and T. R. Malek. 1984. J. Immunol. 133:1970.|4. Malek, T. R., J. A. Schmidt, and E. M. Shevach. 1985. J. Immunol. 134:2405.|5. Rolink, A., U. Grawunder, T.H. Winkler, H. Karasuyama, and F. Melchers. 1994. Int. Immunol. 6:1257.|6. Chen, J., A. Ma, F. Young, and F. W. Alt. 1994. International. Immunology 6:1265.|7. Habu, S., K. Okumura, T. Diamantstein, and E. M. Shevach. 1985. Eur. J. Immunol. 15:456.|8. Moreau, J. L., M. Nabholz, T. Diamantstein, T. R. Malek, E. Shevach, and J. Theze. 1987. Eur. J. Immunol. 17:929.|9. Lorenzo, F., C. Jaulin, N. Vita., P. Froussard, P. Ferrara, D. Jankovic , and J. Theze. J. Immunol. 147:2970.USBio References
No references available