Mouse Anti-CD27 (CD27 Antigen, CD27 Molecule, CD27L Receptor, S152, T-cell Activation Antigen CD27, T14, Tp55, Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7) (FITC)
CD27 is a transmembrane 55kD protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32kD form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 20ul labels 10e6 cells in 100ul suspension or whole blood Optimal working dilutions to be determined by researcher.
Excitation Laser
blue (488 nm)
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human peripheral blood lymphocytes
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by size-exclusion chromatography.
Specificity
Recognizes human CD27.
