Technical Data

C2380-14C
Clone Type
Monoclonal
Host
Mouse
Source
Chicken
Conjugate
PE
Isotype
IgG1,k
Clone Number
AV7
Grade
Purified
Applications
FC IP WB
Crossreactivity
Ch
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Mouse Anti-CD28 (PE)

Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral gamma/delta+ T cells are CD28-negative.1 Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both Vbeta1- and Vbeta2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3.2

Applications
• Flow cytometry 1 • Immunoprecipitation 1 • In vitro T-cell costimulation 2
Recommended Dilution
Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE).
Purity
Purified
Specificity
Recognizes chicken CD28, a monomeric molecule of Mr 40-44kD with 50% amino acid sequence identity with mammalian CD28. Also reacts with turkey CD28 as demonstrated by flow cytometry and immunoprecipitation/Western blot.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Young, J.R., T.F. Davison, C.A. Tregaskes, M.C. Rennie, and O. Vainio. 1994. J. Immunol. 152:3848.|2. Arstila, T.P., O. Vainio, and O. Lassila. 1994. Scand J. Immunol. 40:368.
USBio References
No references available
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