C2380-18A
Clone Type
MonoclonalHost
HamsterSource
MouseIsotype
IgG2Clone Number
37.51Grade
PurifiedApplications
FC IPCrossreactivity
MoShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Hamster Anti-CD28 (CD28 Antigen, CD28 Molecule, MGC138290, T cell Antigen CD28, T cell Specific Surface Glycoprotein, T cell Specific Surface Glycoprotein CD28, Tp44)
CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. 1-4 CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses.5-8
Applications
• Flow cytometry 2-5 • Immunoprecipitation 2 • In vitro costimulation of T cells and NK cells 1, 4, 7
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
C57BL/6N mouse T cell lymphoma EL-4 cell line
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Specificity
Recognizes CD28 costimulatory receptor, Mr 40kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Gross, J. A., E. Callas, and J. P. Allison. 1992. J. Immunol. 149:380.|2. Giese, T., J. P. Allison, and W. F. Davidson. 1993. J. Immunol. 151:597.|3. Clements, J. L., G. Winslow, C. Donahue, S. M. Cooper, J. P. Allison, and R. C. Budd. 1993. Int. Immunol. 5:1451.|4. Nandi, D., J. A. Gross, and J. P. Allison. 1994. J. Immunol. 152:3361.|5. June, C.H., J.A. Bluestone, L.M. Nadler, and C.B. Thompson. 1992. Immunol. Today 15:321.|6. Gelfanov, V., Y. G. Lai, V. Gelfanova, J. Y. Dong, J. P. Su, and N. S. Liao. 1995. J. Immunol. 155:76.|7. Harding, F. A., J. G. McArthur, J. A. Gross, D. H. Raulet, and J. P. Allison. 1992. Nature 356:607.|8. Bluestone, J.A. 1995. Immunity 2:555.USBio References
No references available